Human

Goat

ELISA

Albumin

2-8C

Plasma, Serum, Urine

6.25 ng/ml - 200 ng/ml

1.900 ng/ml

100 min.

The principle of the double antibody sandwich Human Albumin ELISA is represented in Figure 1. In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alb antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ALB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alb in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. The quantity of Alb in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.